作者用问号钩端螺旋体脂多糖(LPS)及其多糖部分(PS)分别免疫仓鼠和豚鼠,用显微镜凝集试验(MAT)检测抗体应答和获得免疫保护所需的最小抗原量,并探讨了LPS和PS作为菌苗的可能性.钩端螺旋体LPS和PS从哥本哈根血清型钩端螺旋体H_(45)株中提取,高压灭菌PS经121℃15分钟高压制备.把40～70g的金色仓鼠随机分成12组,每组4只,用2.5～100μg含有或不含有50μg胞壁酰二肽(MDP)的LPS或PS皮下免疫.用高压灭菌或未经 The authors immunized hamsters and guinea pigs with LPS and polysaccharide fractions (PS), respectively. The antibody response and the minimum amount of antigen required for immune protection were measured by microscopic agglutination test (MAT), and the effects of LPS and PS as a bacterin, Leptospira LPS and PS were extracted from the L. helveticus strain H_ (45) and autoclaved PS was autoclaved at 121 ° C for 15 minutes.The 40-70 g golden hamsters were randomly divided into Twelve groups, 4 in each, were subcutaneously immunized with 2.5 to 100 [mu] g LPS or PS with or without 50 [mu] g muramyl dipeptide (MDP)