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为探讨盐胁迫对丹参种子萌发及幼苗生长过程中生理指标的影响,采用Na Cl模拟不同程度的盐胁迫,共设置0、20、50、75、100和150 mmol·L-1共6个浓度梯度,研究不同程度盐胁迫下丹参种子萌发及幼苗生长的相关生理特性。结果表明,20 mmol·L-1的Na Cl可促进丹参种子萌发,但随着盐胁迫浓度的增加,丹参种子发芽率和发芽指数、发芽势均呈下降趋势;丹参幼苗苗高和根长生长则在75 mmol·L-1 Na Cl溶液处理下达到最大值,且此时显著增加了丹参幼苗叶片中SOD、POD、CAT酶的活性;在100 mmol·L-1 Na Cl溶液处理下可溶性糖,可溶性蛋白含量最高,MDA的含量则随着Na Cl胁迫浓度的升高而增加,说明丹参幼苗叶片通过自身协调物质来抵抗盐胁迫的同时细胞膜随着盐浓度的升高而受到严重的破坏;同时,与对照相比,各处理均显著降低了丹参幼苗的叶绿素与类胡萝卜素的含量,抑制了叶片产生光合色素的能力。综上所述,低浓度的Na Cl胁迫下,有利于丹参种子的萌发,丹参幼苗也可通过提升自身的生理机制来缓解一定浓度盐胁迫造成的伤害,但是随着Na Cl胁迫浓度的升高,丹参幼苗耐盐能力明显下降。
In order to investigate the effects of salt stress on the physiological indexes of Salvia miltiorrhiza seed germination and seedling growth, NaCl concentrations of 0, 20, 50, 75, 100 and 150 mmol·L-1 were used to simulate different levels of salt stress. Gradient to study the physiological and biochemical characteristics of Salvia miltiorrhiza seed germination and seedling growth under different salt stress. The results showed that 20 mmol·L -1 NaCl promoted the germination of Salvia miltiorrhiza. However, the germination rate, germination index and germination energy of Salvia miltiorrhiza were decreased with the increase of salt stress concentration. The seedling height and root length growth of Salvia miltiorrhiza The maximum value was reached under the treatment of 75 mmol·L-1 NaCl solution, and the activities of SOD, POD and CAT in the leaves of Salvia miltiorrhiza seedlings were significantly increased at this time. In the solution treated with 100 mmol·L-1 NaCl, the soluble sugar , The content of soluble protein was the highest while the content of MDA increased with the increase of NaCl concentration, which indicated that Salvia miltiorrhiza seedling leaves could resist the salt stress through their own coordination substances and the cell membrane was seriously damaged with the increase of salt concentration. At the same time, compared with the control, each treatment significantly reduced the chlorophyll and carotenoid content of Salvia miltiorrhiza seedlings and inhibited the ability of the leaves to produce photosynthetic pigments. In summary, the low concentrations of NaCl stress is conducive to the germination of Salvia miltiorrhiza seeds, Salvia miltiorrhiza seedlings can also improve their physiological mechanisms to alleviate the damage caused by a certain concentration of salt stress, but with the Na Cl stress concentration increased Salvia miltiorrhiza seedlings salt tolerance decreased significantly.