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内皮素(endothelin,ET)是一种生物学作用广泛而强烈的缩血管物质,除心血管系统外,肾脏也是重要的效应器官。肾小球系膜细胞(MC)是最活跃的固有细胞,已经证明ET对MC有明显的致收缩和促增殖作用。根据一般规律,MC可能有ET受体(ET-R)。为了证实这一点,我们用逆转录多聚酶链反应(RT-PCR)及Northern印迹杂交法,检测ET-R在MC的表达。 一、材料和方法 1.动物:6～8周龄,体重150～200g SD雄性大鼠。2.MC培养与鉴定:按本实验室已
Endothelin (ET) is a kind of vasoconstrictors with extensive biological effects. In addition to the cardiovascular system, the kidneys are also important effector organs. Glomerular mesangial cells (MC) are the most active innate cells and ET has been shown to cause significant contractile and pro-proliferative effects on MC. According to the general rule, MC may have ET receptor (ET-R). To confirm this, we detected the expression of ET-R in MC by reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot hybridization. First, materials and methods 1. Animals: 6 to 8 weeks old, weighing 150 ~ 200g SD male rats. 2.MC culture and identification: According to the laboratory has been