本文选用30只猫和20只大白鼠进行了放射自显影及高速闪烁自显影联合应用的研究。将~3H—亮氨酸分别注入猫和大白鼠的中枢神经核团内,并严格按照放射自显影神经追踪术和高速闪烁自显影程序制备标本。结果放射自显影神经追踪术具有敏感性高、定位精确、神经元处于生理状态下进行实验等优点。同时结合高速闪烁自显影可早期判断注射位点。关于标记物的注人我们认为以高浓度、小体积,慢匀速注射为好。本文还对动物存活期,冰冻切片等问题进行了讨论。 In this paper, 30 cats and 20 rats were used for autoradiography and high-speed scintigraphy combined study. The ~ 3H-leucine was injected into the CNS of cats and rats, respectively, and the samples were prepared strictly according to autoradiography and high-speed scintigraphy. The results of autoradiography tracing with high sensitivity, accurate positioning, neurons in the physiological state of the experiment and so on. At the same time combined with high-speed flashing self-imaging can be early to determine the injection site. About the injection of markers We think of high concentration, small volume, slow uniform injection as well. This article also discusses the survival of animals, frozen sections and other issues.