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我们用放射免疫分析法测定了40例肝硬化患者及50例健康人血清粒细胞-巨噬细胞集质刺激因子GM-CSF)的含量,以探讨GM-CSF与肝硬化之间的关系,现将结果报道如下。 对象和方法 一、对象:40例(男22,女18),肝硬化患者年龄27～56岁。按全国肝硬化专题讨论会拟定的标准明确诊断肝硬化的住院病人。50例健康人(男25,女25),年龄25～50岁,选自来本院健康体检者。所有受检者均抽晨空腹血分离血清于冰箱保存待测。 二、方法:3.0ml,-20℃ GM-CSF RIA kit由北京华英放射免疫技术研究所提供。仪器为西安262厂生产的XH-6010全自动γ-计数仪。操作按说明书。 结果 肝硬化患者GM-CSF明显低于正常对照组(P<0.001),结果见表1。
We measured the content of GM-CSF in 40 cirrhotic patients and 50 healthy people by radioimmunoassay to investigate the relationship between GM-CSF and cirrhosis The results are reported below. Objects and methods First, the object: 40 cases (male 22, female 18), cirrhosis patients aged 27 to 56 years. According to the National Symposium on Cirrhosis formulated a clear diagnosis of liver cirrhosis inpatients. 50 healthy people (25 males and 25 females), aged 25 to 50 years, were selected from our hospital for physical examination. All subjects were taken morning fasting blood serum was stored in the refrigerator to be tested. Second, the method: 3.0ml, -20 ℃ GM-CSF RIA kit from Beijing Huaying Institute of Radioimmunoassay provided. Instrument for the 262 factory in Xi’an XH-6010 automatic γ-counter. Operation according to the instructions. Results The liver cirrhosis patients with GM-CSF was significantly lower than the normal control group (P <0.001), the results in Table 1.