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目的 研究常规给水处理工艺水中有机提取物致细胞氧化应激的影响.方法 以人胚肝细胞(L-02细胞)为靶细胞,水样有机提取物设1.2、6、30、150ml/ml培养液四个剂量,二甲基亚砜(DMSO,10ml/L)为溶剂对照.H_2O_2(100μmol/L)为阳性对照,染毒L-02细胞24 h后,检测细胞内脂质过氧化产物丙二醛(MDA)、还原型谷胱甘肽(GSH)和8一羟基脱氧鸟苷(8-OHdG)的含量.结果 与溶剂对照相比,给水处理各工艺环节的水样有机提取物在较高浓度(30、150ml/ml培养液)时均使L-02细胞MDA含量增加(P＜0.05,P＜0.01),GSH含量下降(P＜0.05,P＜0.01);各工艺环节中MDA含量的变化趋势是:预氯.混凝沉淀工艺使其增加.沉淀工艺使其降低,加氯消毒工艺使MDA含量再次增加;GSH含量在上述相应工艺环节则表现为先下降,再上升,后又下降的趋势.与溶剂对照相比,各工艺环节水样有机提取物浓度为30 ml/ml培养液时,L-02细胞中8-OHdG水平升高(P＜0.05,P＜0.01),且与MDA的变化趋势一致.结论 两次加氯工艺增强了有机提取物对L-02细胞的氧化应激作用.使脂质过氧化反应增强、抗氧化作用降低、DNA氧化损伤增加.沉淀、过滤工艺可以减弱上述有害效应.“,”Objective To investigate the effects of drinking water treatment procedures on the cellular oxidative stress induced by organic extracts of drinking water. Methods Human embryonic liver cell line (L-02) was treated with water organic extracts of 1.2, 6, 30 and 150 ml water samples per millililer culture. DMSO (10 ml/L) and H_2O_2 (100 μmol/L) were used as the negative and positive controls, respectively. MDA, GSH and 8-OHdG in L-02 cell cultures were determined after 24 h of treatment with water organic extracts. Results The contents of MDA were significantly increased and the contents of GSH were significantly decreased in L-02 cells exposed to organic extracts at the concentrations of 30 and 150 ml water samples/ml culture as compared with solvent control (P＜0.05 or P＜0.01). Changes of MDA level showed tendencies to increase after pre-chlorination plus coagulation-sedimentation and to decrease after precipitation, then to increase again after post-chlorination. The change tendencies of GSH were contrary to those of MDA. Levels of 8-OHdG in L-02 cells exposed to organic extracts at the concentration of 30 ml/ml culture were significantly increased as compared with solvent control (P＜0.05, P＜0.01) and showed the same change tendencies of MDA. Conclusion Both the pre-chlorination and post-chlorination steps lead to the increase of oxidative stress in L-02 cells, but precipitation and filtration show the opposing effects.