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微核试验常用于检测环境理化因子的致突变作用。我们试用于检测生物因子造成的差异,发现处于不同微生物水平下的无菌(GF)大鼠与普通(CV)大鼠之间差异有显著性,推断微生物可能是影响实验动物遗传物质稳定性的原因之一。为了进一步验证这一论点,本实验将Wistar GF大鼠(本所悉生生物室培育)分别转到无特定病原体(SPF)和CV环境下,比较GF、SPF化和CV化大鼠骨髓嗜多染红细胞微核率。实验分3组:1.GF大鼠组,10只;2.SPF化大鼠组,8只;3.CV化大鼠组,7只。饲养条件均相同。经微生物和寄生虫检测符合
Micronucleus tests are often used to detect the mutagenic effects of environmental physicochemical factors. We tested the differences caused by biological factors and found that there was a significant difference between sterile (GF) rats and common (CV) rats at different levels of microorganisms. It was concluded that microorganisms may affect the stability of genetic material of experimental animals. one of the reasons. In order to further verify this argument, Wistar GF rats (cultured in Biology Research Laboratory) were transferred to specific pathogen-free (SPF) and CV environments to compare GF, SPF, and CV rat bone marrow Erythrocyte micronucleus rate. The experiment was divided into three groups: 1. GF rat group, 10; 2. SPF rat group, 8; 3. CV rat group, 7. The feeding conditions are the same. Conformity with microbiological and parasite detection