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高效液相色谱(HPLC)被广泛认为是分离制备光学纯单一对映体最有效的方法。在高效液相色谱手性拆分中,手性固定相的性能直接影响到色谱柱的手性分离能力。在众多手性固定相中,键合型手性固定相具有溶剂耐受性好,分离模式灵活等优点,是很重要的一大类手性固定相。本文主要针对大分子键合型手性固定相,包括多糖衍生物键合型手性固定相、蛋白质键合型手性固定相以及合成高分子键合型手性固定相,就其柱负载、溶剂耐受性、分离效果等方面,综述了大分子键合型手性固定相的研究进展。
High performance liquid chromatography (HPLC) is widely considered as the most efficient method for the separation of optically pure enantiomers. In chiral resolution of HPLC, the performance of chiral stationary phase directly affects the chiral separation ability of the column. Among the many chiral stationary phases, the bonded-type chiral stationary phases have the advantages of good solvent resistance and flexible separation modes, and are very important class of chiral stationary phases. In this dissertation, macromolecule-bonded chiral stationary phases, including polysaccharide derivative-bonded chiral stationary phases, protein-bound chiral stationary phases, and synthetic polymeric bonded chiral stationary phases, Solvent tolerance, separation effect, etc., reviewed the research progress of macromolecule bonded chiral stationary phase.