利用微生物转化的方法,以Bacillus subtilisYD 016为转化菌株,对二氢去氢双松柏醇的转化进行研究。该菌株可以将二氢去氢双松柏醇完全转化,主要得到3种转化产物(记为HMZG-1、HMZG-2、HZMG-3)。通过硅胶柱层析和制备型HPLC方法对转化产物进行分离纯化,经ESI-MS、~1H-NMR和~(13)C-NMR分析,转化产物分别鉴定为二氢去氢双松柏醇-4-O-β-D-吡喃葡萄糖苷(HMZG-1)、二氢去氢双松柏醇-9’-O-β-D-吡喃葡萄糖苷(HMZG-2)、二氢去氢双松柏醇-9-O-β-D-吡喃葡萄糖苷(HMZG-3)。通过高效液相色谱法对生物转化情况进行检测,建立转化产物和底物的标准曲线。根据HPLC结果计算可知,3种转化产物的转化率分别为:17.34%,14.29%,66.41%。该研究丰富了Bacillus subtilis YD 016的底物作用范围,为二氢去氢双松柏醇葡萄糖苷的合成提供了一条新途径。 The transformation of dihydrogen dehydrogenated double-coniferyl alcohol was studied by using the method of microbial transformation and Bacillus subtilisYD 016 as the transformed strain. This strain can completely convert dihydro-dehydrogenated coniferyl alcohol, resulting in three major transformation products (denoted as HMZG-1, HMZG-2 and HZMG-3). The conversion products were separated and purified by silica gel column chromatography and preparative HPLC. The products were identified as dihydro-dehydro-disintegrin-4 O-β-D-glucopyranoside (HMZG-1), dihydro-dehydro-disintegrin-9’-O-β-D-glucopyranoside (HMZG-2) Alcohol-9-O-β-D-glucopyranoside (HMZG-3). Bioconversion was detected by HPLC and a standard curve of the conversion product and substrate was established. According to the calculation of HPLC results, the conversion rates of the three conversion products are respectively 17.34%, 14.29% and 66.41%. This study enriched the substrate range of Bacillus subtilis YD 016 and provided a new way for the synthesis of dihydrogen-dehydro double coniferyl glucoside.