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背景:越来越多的研究事实表明免疫系统不是一个仅有自主调节的孤立系统,而是与中枢神经系统之间存在双向联系的,但中枢神经系统哪些部位参与免疫调节仍是一个尚未解决的问题。目的:研究与神经免疫调节有关的功能神经元在大鼠脑内的分布。设计:随机对照的研究。单位:解放军第一军医大学珠江医院神经外科,解放军第四军医大学神经科学研究所和解放军第四军医大学唐都医院呼吸科。对象:实验在第一军医大学珠江医院神经外科和第四军医大学全军神经科学研究所完成。10只健康清洁级成年SD大鼠由第四军医大学实验动物中心提供。干预:以腹腔注射脂多糖(LPS)为免疫激发模型,采用免疫组织化学抗Fos蛋白ABC方法进行染色。主要观察指标:Fos蛋白在脑内不同区域内的分布。结果:Fos阳性产物多集中分布在大脑额顶皮质、边缘前脑(扣带皮质、梨状皮质、外侧隔核和中央杏仁核等)、丘脑室旁核、下丘脑室旁核、弓状核、视上核、视交叉上核、下丘脑外侧区、中脑导水管周围灰质腹外侧部、外侧臂旁核和延髓内脏带。小脑内无明显Fos分布密集区。结论:LPS诱发的Fos阳性神经元在脑内有相对广泛的分布。
BACKGROUND: A growing body of research shows that the immune system is not an isolated, autonomous system but a two-way relationship with the central nervous system. However, it is still not clear which parts of the central nervous system are involved in immune regulation problem. AIM: To investigate the distribution of functional neurons involved in neuroimmunity regulation in rat brain. Design: A Randomized Controlled Study. Unit: Department of Neurosurgery, Zhujiang Hospital, First Military Medical University of PLA, Institute of Neuroscience, Fourth Military Medical University of Chinese PLA, and Department of Respiratory Diseases, Tangdu Hospital, Fourth Military Medical University, People’s Liberation Army. PARTICIPANTS: Experiments were performed at Department of Neurosurgery, Zhujiang Hospital, First Military Medical University and Institute of Neurological Science, Fourth Military Medical University. Ten healthy and clean adult SD rats were provided by Experimental Animal Center of the Fourth Military Medical University. Intervention: Lipopolysaccharide (LPS) was immunized by intraperitoneal injection and immunohistochemistry was used to stain Fos protein ABC. MAIN OUTCOME MEASURES: Distribution of Fos protein in different regions of the brain. Results: Fos positive products were mainly distributed in the frontal cortex, marginal forebrain (cingulate cortex, piriform cortex, lateral septal nucleus and central amygdala, etc.), paraventricular nucleus of hypothalamus, paraventricular nucleus of hypothalamus, arcuate nucleus , Supraoptic nucleus, suprachiasmatic nucleus, lateral hypothalamic area, midbrain periaqueductal gray, lateral parabrachial nucleus and medullary visceral zone. No obvious Fos distribution in cerebellum. Conclusion: LPS-induced Fos-positive neurons have a relatively broad distribution in the brain.