本文记述了一种生物测定方法,能有效的用来测定昆虫病原真菌—白僵菌分生孢子制剂Beauveia bassiana(Bals)vuill,对取食用不同浓度的分生孢子表面处理叶盘上的粉纹夜蛾Trichoplusia ni(Hubner)一龄幼虫的毒力。48小时后,幼虫转移到未处理的半合成饲料上(温室25±1℃),五天后终止测定。这种生物测定的剂量—死亡率回归方程是:Y=2.1420+1.3352X。计算得出的致死浓度Lc20、Lc50和Lc80分别为每平方毫米上总分生孢子数32,139和590个。 This article describes a bioassay method that can be used effectively to determine Beauveia bassiana (Bals) vuill, an entomopathogenic fungus of the entomopathogenic fungus, on the surface of the leaf disc on condiae treated with different concentrations of conidia Toxicity of the first instar larvae of Trichoplusia ni (Hubner). After 48 hours, larvae were transferred to untreated semi-synthetic feed (greenhouse 25 ± 1 ° C) and the assay was discontinued five days later. The dose-mortality regression equation for this bioassay was: Y = 2.1420 + 1.3352X. The calculated lethal concentrations of Lc20, Lc50 and Lc80 were 32,139 and 590 conidia per square millimeter, respectively.