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目的 研究催乳素对Graves病 (GD)甲状腺细胞与自体外周血单个核细胞 (PBMC)在体外共同培养时相互作用的影响。方法 利用免疫荧光染色和流式细胞仪等 ,测定在不同羊催乳素(oPRL)水平进行共同培养时PBMC的活化、增殖反应和甲状腺细胞对人类白细胞抗原基因复合体(HLA) DR及CD4 0 的表达。结果 oPRL浓度为 2 0 0 μg/L时与GD甲状腺细胞共同培养的PBMC中CD4 +CD2 5+细胞百分率 [(13 0 8± 2 5 4) % ,P <0 0 1]和增殖指数 [(17 82± 3 0 2 ) % ,P <0 0 1]及 10 0 0μg/L时的增殖指数 [(16 5 7± 2 5 6 ) % ,P <0 0 5 ]均较 0 [分别 (10 15± 2 6 0 ) %和 (14 38± 2 6 4) % ]、12 5及 5 0 μg/L时有显著增加。在 2 0 0和 10 0 0 μg/L时相应甲状腺细胞中CD4 0 +细胞百分率 [(4 8 2 5± 6 6 3) % ,(5 2 2 8± 6 94) % ]和平均荧光强度 (dMF ;42 94± 10 2 4,49 5 1± 12 34)明显低于 0 [(5 8 38± 6 6 2 ) %和 6 7 30± 2 0 2 0 ]、12 5及 5 0 μg/L剂量组。而在 5 0 μg/L时其HLA DR+细胞百分率[(4 6 79± 7 5 1) % ,P <0 0 1]和dMF(2 1 0 2± 5 43 ,P <0 0 1)显著高于 0 [(33 5 1± 8 5 8) %和 13 91±3 88]、12 5、2 0 0及 10 0 0 μg/L剂量组。除此以外 ,oPRL各剂量组间差异均
Objective To study the effect of prolactin on the interaction of thyrocytes with autologous peripheral blood mononuclear cells (PBMC) in Graves’ disease (GD) in vitro co-culture. Methods Immunofluorescence staining and flow cytometry were used to detect the activation and proliferation of PBMC and the effect of thyroid cells on the HLA DR and CD4 0 levels in co-cultures of different sheep prolactin (oPRL) expression. Results The percentage of CD4 + CD25 + cells in PBMC co-cultured with GD thyroid cells at oPRL concentration of 200 μg / L [(13 0 8 ± 2 54)%, P 0 01) and proliferation index [( 17 82 ± 3 0 2%, P 0 01, and proliferation index at 100 microg / L were (16 57 ± 25 56)%, P 0 05, respectively 15 ± 2 6 0% and (14 38 ± 2 6 4)%] at 12 5 and 50 μg / L, respectively. The percentages of CD4 0 + cells [(4852 ± 6 6 3)%, (5282 ± 6 94)%] and mean fluorescence intensities in the corresponding thyroid cells at 200 and 100 μg / L dMF; 42 94 ± 10 2 4,49 5 1 ± 12 34) was significantly lower than 0 [(58 8 ± 6 6 2)% and 6 7 30 ± 20 2 0], 12 5 and 50 μg / L Dose group. While the percentage of HLA DR + cells at the level of 50 μg / L [(469 ± 751)%, P <0.01] and dMF (2 102 ± 5 43, P <0.01) were significantly higher At 0 [(33 5 ± 8 58)% and 13 91 ± 3 88], 125, 200 and 100 μg / L doses, respectively. In addition, oPRL differences between the various dose groups were