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目的:研究人心房肽(hANP)cDNA转染细胞的微囊化技术中心房肽分泌的近日节律;通过人工调控,改变心房肽分泌的近日节律,达到有效治疗高血压或心力衰竭的目的。方法:将人心房肽基因(cDNA)转染入中国仓鼠卵巢细胞(Chineseham-sterovary,CHO),然后将细胞包被在聚己内酯管内形成微囊,并检测转基因CHO细胞长时间存活情况和分泌的心房肽。检测微囊化转基因细胞分泌心房肽的生物节律,并用褪黑素进行调整。结果:在培养期间,长度20mm,直径2mm的聚己内酯管内转染CHO细胞在2mL培养基中24h分泌的心房肽水平可达210.3ng/L;心房肽的分泌呈近日节律性变化,日间高,而夜间低;近日节律变化的时相是4:15,用褪黑素处理,近日节律的时相移至7:55。结论:心房肽cDNA转染的CHO细胞包被在聚己内酯管内并能向管外分泌心房肽,将来可能适于植入人体。此项研究为心房肽治疗高血压或心力衰竭提供了一条新途径。
OBJECTIVE: To study the recent rhythm of atrial natriuretic peptide secreted by microencapsulated technology in human atrial natriuretic peptide (hANP) -transfected cells and to change the current rhythm of atrial natriuretic peptide secretion by artificial regulation so as to effectively treat hypertension or heart failure. Methods: The human atrial natriuretic peptide (cDNA) was transfected into Chinese hamster ovary cells (CHO). The cells were then coated with polycaprolactone to form microcapsules. The long-term survival of transgenic CHO cells Secreted atrial peptide. The biological rhythms of atrial natriuretic peptides secreted by microencapsulated transgenic cells were examined and adjusted with melatonin. RESULTS: CHO cells transfected with polycaprolactone with a length of 20 mm and a diameter of 2 mm were cultured in 2 mL culture medium for 24 h, and the atrial natriuretic peptide secretion reached 210.3 ng / L during the culture period. The secretion of atrial natriuretic peptide Between high, while the night is low; recent rhythm changes in the time is 4:15, with melatonin treatment, the rhythm of the recent phase shift to 7:55. CONCLUSION: Atrial natriuretic peptide cDNA-transfected CHO cells are encapsulated in polycaprolactone tubes and capable of secreting atrial natriuretic peptides outside the tube and may in the future be suitable for implantation into the human body. This study provides a new avenue for the treatment of atrial fibrillation with high blood pressure or heart failure.