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目的研究熊胆粉在麝香通心滴丸抗动脉粥样硬化中的作用,为其凉开法治疗特点及临床推广应用提供实验数据。方法采用特异性基因敲除小鼠结合高脂饮食复制动脉粥样硬化动物模型,灌服熊胆粉水溶液,并以麝香通心滴丸全方水溶液及全方去熊胆粉水溶液为对照,通过HE染色、油红O染色及Masson’s Trichrome染色,观察对主动脉及主动脉根动脉粥样硬化斑块形成的干预作用。生化法测定血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)及低密度脂蛋白(LDL)水平。结果与正常组对比,模型组斑块损伤面积显著增加(P<0.01),血清TG、TC和LDL均显著升高(P<0.01),HDL明显下降(P<0.01);与模型组对比,各给药组斑块损伤面积均显著减小(P<0.05),全方组作用优于去熊胆粉组。各给药组与模型组对比,血清TG、TC均显著下降(P<0.01),HDL明显上升(P<0.01),全方组作用优于去熊胆粉组,此外,熊胆粉组降低LDL作用显著(P<0.01)。结论熊胆粉在麝香通心滴丸抗动脉粥样硬化作用中具有突出的贡献,特别是在降低LDL作用中贡献显著。
Objective To study the role of Xiongdi powder in the treatment of atherosclerosis of musk Tongxin pills and to provide experimental data for the characteristics and clinical application of its treatment. Methods Specific knockout mice combined with high-fat diet to replicate atherosclerotic animal model were orally administered with Xiong GTI powder aqueous solution. HE staining, oil red O staining and Masson’s Trichrome staining were used to observe the intervention effects on atherosclerotic plaque in the aorta and aortic root. The levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) in serum were determined by biochemical method. Results Compared with the normal group, the lesion area of plaque in model group increased significantly (P <0.01), the levels of TG, TC and LDL in serum increased significantly (P <0.01) and HDL decreased significantly (P <0.01) The area of plaque damage in each administration group was significantly reduced (P <0.05), and the effect of the whole prescription group was better than that of the bear gall gall powder group. Compared with the model group, the TG and TC in serum of all groups were significantly decreased (P <0.01) and HDL was significantly increased (P <0.01), and the effect of all prescriptions was better than that of Xiongxiong bolus. In addition, LDL effect was significant (P <0.01). Conclusion Bear gall powder has a prominent contribution to the anti-atherosclerosis effect of Shexiang Tongxin Dripping Pills, especially in reducing the effect of LDL.