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为了探讨B7-H1分子诱导产生的T抑制细胞免疫生物学特性,我们首先构建了人B7-H1基因,筛选出细胞膜表面稳定表达B7-H1蛋白的人ECV304细胞系,并在体外建立了由这种非专职抗原提呈细胞系刺激诱导纯化的CD4+T细胞,并使其具有抑制功能的新方法。通过检测其增殖效应和上清细胞因子水平的变化,观察到这群细胞对同种异体混合淋巴细胞反应(MLC),具有明显抑制作用,该作用与大量产生IL-10、TGF-β细胞因子有关。实验表明,ECV304/B7-H1可有效诱导具有免疫抑制的功能T细胞,为其进一步应用于临床治疗打下基础。
In order to investigate the biological characteristics of T-suppressor cells induced by B7-H1, we first constructed the human B7-H1 gene and screened the human ECV304 cell line stably expressing B7-H1 on the cell surface. A nonprofessional antigen presenting cell line stimulates a new method of inducing purified CD4 + T cells and rendering them functionally inhibitory. By detecting the proliferation effects and the changes of the supernatant cytokines, we observed that this group of cells had a significant inhibitory effect on allogeneic mixed lymphocyte reaction (MLC). This effect was associated with a large number of production of IL-10, TGF-β cytokines related. Experiments show that, ECV304 / B7-H1 can effectively induce immunosuppressive function of T cells, which laid the foundation for its further application in clinical treatment.