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目的:在分析区域包含整个颅顶骨的前提下,改变颅顶骨显微CT(micro-computed tomography,Micro-CT)扫描后二维图像的重建层数或者位置,比较进行重建后的骨量参数(骨表面积、骨体积)是否出现改变。方法:4日龄小鼠颅顶骨培养后置于含培养液的EP管中,进行连续Micro-CT扫描。根据重建时改变层数或位置来分别分析:(1)996~22层组与800~50层组,分析层数不同(n=3);(2)996~22层组与随机选择层组,分析层数不同(n=3);(3)近边界上缘组与近边界下缘组,分析层数相同(n=3)的骨量参数变化。结果:在分析区域包含整个颅顶骨的前提下:(1)增加或者减少层数导致骨表面积变化(F=47.798,P=0.002),而骨体积无变化(F=7.083,P=0.056)。(2)随机选择层数组与996~22层数组分析比较时骨表面积(F=359.392,P=0.000)、骨体积(F=51.844,P=0.002)均有变化。(3)重建层数相同的前提下,改变分析区域位置时骨表面积(F=0.000,P=0.991)、骨体积(F=0.000,P=0.995)无变化。结论:(1)Micro-CT三维重建时,改变重建层数可影响骨量参数。(2)Micro-CT三维重建时,随机选择重建层数可影响骨量参数。(3)Micro-CT三维重建时,保证分析层数相同而改变空白分析区域的位置不会影响骨量参数。
OBJECTIVE: To change the reconstructed layer number or position of the two-dimensional image after micro-computed tomography (Micro-CT) scan of the skull under the premise of analyzing the entire parietal bone in the analysis area and to compare the bone mass parameters Bone surface area, bone volume) is changed. Methods: The 4-day-old mice were cultured in the parietal bone and placed in the EP tube containing the culture medium for continuous Micro-CT scanning. According to the change of layer or location during reconstruction, we analyzed separately: (1) 996 ~ 22 layers and 800 ~ 50 layers, with different layers (n = 3); (2) (N = 3); (3) The upper boundary group near the border and the lower margin group near the border were used to analyze the changes of the bone mass parameters with the same number of layers (n = 3). RESULTS: In the analysis of the entire parietal bone, (1) increasing or decreasing the number of layers resulted in a change in the surface area of the bone (F = 47.798, P = 0.002) without any change in the bone volume (F = 7.083, P = 0.056). (2) The bone surface area (F = 359.392, P = 0.000) and the bone volume (F = 51.844, P = 0.002) all changed when the random selection layer array was compared with the 996 ~ 22 layer array analysis. (F = 0.000, P = 0.991), bone volume (F = 0.000, P = 0.995) did not change under the same reconstruction layer number. Conclusion: (1) Micro-CT three-dimensional reconstruction, the change in the reconstruction of the number of layers can affect bone parameters. (2) When Micro-CT three-dimensional reconstruction, randomly select the reconstruction layer can affect the bone mass parameters. (3) Micro-CT three-dimensional reconstruction, to ensure the same analysis of the same number of layers to change the position of the blank analysis area will not affect the bone mass parameters.