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为了解小麦条锈病抗病基因在染色体上的位置,对源自小麦杂交组合宁7840×Clark的重组自交系(RIL)群体进行了抗条锈病QTL分析。结果表明,在染色体1BS上检测到一个主效的QTL即QYr-hwwg-1B。该QTL由抗病亲本宁7840提供,位于SNP标记Xsnp3620和Xsnp5435之间,区间长度为2.5cM,可解释55.8%的表型变异。根据宁7840的小种抗性推测QYr-hwwg-1B可能是由来自1B/1R易位系的抗病基因Yr9引起的。抗性基因Yr9、Yr10、Yr15、Yr24、Yr26、YrH52和YrAlp均位于小麦1B染色体短臂的一端,形成一个抗条锈基因簇,并与SSR标记Xgwm11紧密连锁。另外,有56个SNP标记与该标记区间共分离,可以用于小麦抗条锈基因精细定位图谱的构建及分子标记辅助选择育种。
To understand the chromosomal location of disease resistance genes in wheat stripe rust, a QTL analysis of resistance to stripe rust was conducted in a population of recombinant inbred lines (RILs) derived from the cross of Ning7840 × Clark. The results showed that a major QTL for QYr-hwwg-1B was detected on chromosome 1BS. The QTL was provided by the resistant parent Ning 7840, located between the SNP markers Xsnp3620 and Xsnp5435 with a length of 2.5 cM, accounting for 55.8% of phenotypic variation. It was speculated that QYr-hwwg-1B might be caused by the resistance gene Yr9 from 1B / 1R translocation line. The resistance genes Yr9, Yr10, Yr15, Yr24, Yr26, YrH52 and YrAlp were located at one end of the short arm of 1B chromosome of wheat, forming a cluster of stripe rust resistance gene and closely linked with SSR marker Xgwm11. In addition, 56 SNP markers were co-segregated with this marker region and could be used for the construction of fine mapping map of wheat stripe rust resistance and molecular marker-assisted selection breeding.