金雀异黄素抑制SGC7901细胞增殖、迁移和粘附的研究

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目的 探讨金雀异黄素(Gen)对人胃腺癌SGC7901细胞增殖、迁移以及与血管内皮细胞粘附的影响。方法 利用细胞计数和MTT方法检测Gen对SGC7901细胞增殖的影响;利用细胞划痕实验检测Gen对SGC7901细胞迁移的影响;在单层脐静脉内皮细胞上加入SGC7901细胞,检测Gen对SGC7901细胞与血管内皮细胞粘附的影响;利用免疫荧光实验观察Gen对SGC7901细胞中E-cadherin、β-catenin和肌动蛋白(actin)的分布的影响;利用Westernblotting检测Gen对SGC7
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