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用人乳头瘤病毒（HPV）高度保守的通用引物和16、18型特异引物对123例给直肠组织作聚合酶链反应（PCR）检测HPVDNA，总检出率为14．60％正常粘膜、结肠炎和炎性息肉组为3．3％（2／71），乳头状腺瘤为17．6％（3／17），原位癌和浸润癌为37．1％（13／35）．在正常组织、癌旁组织和癌组织HPVDNA阳性率分别为2．9％、5．7％和37.1％．在结直肠腺癌中HPV感染以18型多见，18型与16型之比为3:1，HPV在左半结肠以下比右半结肠感染率高。
HPVDNA was detected by polymerase chain reaction (PCR) in 123 cases of rectal tissues with the universal primers highly specific to human papilloma virus (HPV) and type-specific primers 16 and 18. The overall detection rate was 14.60%. Normal mucosa and colitis And inflammatory polyp group were 3.3% (2/71), papillary adenoma was 17.6% (3/17), in situ carcinoma and invasive carcinoma was 37.1% (13/35). The positive rates of HPVDNA in normal tissues, paracancerous tissues and cancerous tissues were 2.9%, 5.7% and 37.1% respectively. HPV infection in colorectal adenocarcinoma more common type 18, 18 type and 16 type ratio of 3: 1, HPV in the left lower colon than the right colon infection rate.