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目的探讨1-溴丙烷(1-BP)对大鼠大脑离子型谷氨酸受体NR2B亚基和GluR2亚基的影响。方法 无特定病原体级成年雄性Wistar大鼠36只,随机分为对照组、低剂量组、中剂量组和高剂量组4组,于动式吸入染毒柜内分别暴露于质量浓度为0、1 250、2 500和5 000 mg/m3的1-BP气体,8 h/d,每周染毒5 d,连续4周。实验结束后处死大鼠,分离脑组织,应用蛋白质印迹法和实时荧光定量聚合酶链式反应法测定NR2B和GluR2的蛋白和基因表达水平。结果 中、高剂量组大鼠大脑NR2B和GluR2的蛋白表达水平均高于对照组和低剂量组(P<0.05或P<0.01)。与对照组相比,高剂量组大鼠大脑NR2B mRNA表达水平增加(P<0.05),各剂量组大鼠大脑GluR2 mRNA表达水平均增加(P<0.05或P<0.01)。结论 一定水平的1-BP亚急性吸入染毒能增加大鼠大脑NR2B和GluR2的蛋白表达。
Objective To investigate the effects of 1-bromopropane (1-BP) on NR2B and GluR2 subunits of glutamate receptors in rat brain. Methods Thirty-six adult male Wistar rats without specific pathogen were randomly divided into 4 groups: control group, low dose group, middle dose group and high dose group. The animals were exposed to a concentration of 0,1 1-BP gas at 250, 2 500 and 5 000 mg / m3 for 8 h / d and weekly for 5 days for 4 weeks. After the experiment, the rats were sacrificed and the brain tissues were separated. The protein and gene expression of NR2B and GluR2 were determined by Western blotting and real-time fluorescence quantitative polymerase chain reaction. Results The protein levels of NR2B and GluR2 in the high-dose and high-dose groups were higher than those in the control group and the low-dose group (P <0.05 or P <0.01). Compared with the control group, the expression of NR2B mRNA in the brain of high-dose group increased (P <0.05), and the expression of GluR2 mRNA increased (P <0.05 or P <0.01) in each dose group. Conclusion A certain level of 1-BP subacute inhalation can increase the protein expression of NR2B and GluR2 in rat brain.