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目的:观察扎里奴思方对脑缺血再灌注损伤后大鼠脑内神经生长因子(NGF)表达的影响。方法:线栓法制备局灶性脑缺血再灌注大鼠模型。实验动物随机分为假手术组、模型组、扎里奴思方组、尼莫地平组。取材前进行神经功能评分。术后各组分别在1,3,7,14 d各个时间点取材,HE染色法观察大鼠脑组织病理学改变;免疫组织化学法测定大鼠脑内NGF的表达。结果:模型组大鼠神经功能评分降低,神经元数量减少,大小不均,病理损伤显著;与模型组比较,尼莫地平和扎里奴思方组神经评分增高,神经元数量增多,脑组织病理损伤减轻明显,NGF表达增高(P<0.01,P<0.05);与尼莫地平组比较,扎里奴思方14 d组神经功能评分和NGF表达明显升高(P<0.05)。结论:扎里奴思方可对局灶性脑缺血再灌注大鼠脑组织神经元起保护作用,扎里奴思方14天组的脑保护作用更强,其脑保护作用机制可能与调节大鼠脑组织NGF表达有关。
Objective: To observe the effect of Zarimus on nerve growth factor (NGF) expression in rat brain after cerebral ischemia-reperfusion injury. Methods: The rat model of focal cerebral ischemia / reperfusion was established by suture method. Experimental animals were randomly divided into sham operation group, model group, Zarimus prescription group and nimodipine group. Neurological score before taking. The rats in each group were taken at 1, 3, 7, and 14 days after operation, respectively. The pathological changes of brain were observed by HE staining. The expression of NGF in rat brain was detected by immunohistochemical method. Results: Compared with the model group, the neurological score, the number of neurons and the number of neurons in the nimodipine and Zarimus group increased significantly (P <0.01, P <0.05). Compared with nimodipine group, neurological scores and NGF expression in 14-day Zarimus group were significantly increased (P <0.05). Conclusion: Zarimus can protect the neurons in brain of focal cerebral ischemia-reperfusion rats. The protective effect of Zarimus prescription for 14 days group is stronger than that of control group NGF expression in rat brain tissue.