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将 5 m m× 5 m m的香料烟叶块接种到 2 / 3MS+BA0 .5 mg/ L +IAA0 .1mg/ L的培养基中培养 ,4周后分化出大量丛生芽。继续培养 2周后 ,芽长成苗高 1.0～ 1.5 cm,形成 5片小真叶时 ,转移到 2 / 3MS+BA0 .1m g/ L+IAA1的培养基中分化出根 ,形成完整的再生植株。当植株高 2 .5～ 3.5 cm,具有 6～ 7片小真叶时 ,移栽到经过灭菌的营养土盆中。开始时先用 1/ 3MS培养液浇苗 1d,以后正常管理 ,定植后营养生长 2周形成 8片真叶进入团棵期 ,再继续营养生长 4周 ,有效叶 9片时开始现蕾 ,10 d后开花 ,并相继结出绿蒴果。开花后 30 d果实成熟为褐色蒴果
The 5 m m × 5 m m tobacco leaves were inoculated into 2/3 MS + BA0.5 mg / L + IAA0.1 mg / L medium and cultured for 4 weeks. After two weeks of continuous culture, shoots grew to 1.0-1.5 cm in height. When five small true leaves were formed, the shoots were transferred to 2 / 3MS + BA0.1m g / L + IAA1 and the roots were differentiated to form a complete regeneration Plant. When the plant height 2.5 ~ 3.5 cm, with 6 to 7 small true leaves, transplanted into the sterilized nutrient basin. Initially, 1 / 3MS culture solution was used to seed the seedlings for 1 day and then be managed normally. After vegetative planting, vegetative growth for 2 weeks resulted in 8 true leaves entering the Tuanchushu period and then continued vegetative growth for 4 weeks. After d flowering, and have come to green capsules. Fruit ripened to brown capsules 30 d after flowering