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我科以人型结核菌 PPD-C、BCG-PPD 和植物血球凝集素(PHA)三联皮内注射,进行结核病的免疫诊断,已应用多年,现随机选择168例观察结果报告如下。1 资料与方法1.1 PHA 系广州医药工业研究所提供的冻干注射液。以生理盐水稀释,于右前臂屈侧皮内注射0.1ml(20ug)。注射后皮丘直径为7mm～9mm;24小时再测量,若皮丘直径增大或无变化为(+),减小为(±),消失为(-)~(〔1〕)。1.2 人型结核菌 PPD-C 系北京高科生命科学技术开发公司生产的稀释液。皮内注射0.1ml(50IU/ml)于左前臂屈侧。结果判断以72小时局部肿结直径为依据;凡肿结直径≤4mm为无反应(-),5mm～10mm 为阳性反应(+),11mm～20mm为中度阳性反应(++),>20mm 或虽然不超过此值但有水泡,坏死,为强阳性反应(+++)~(〔2〕)。
Mycobacterium tuberculosis PPD-C, BCG-PPD and phytohemagglutinin (PHA) triple intradermal injection of tuberculosis for immune diagnosis, has been used for many years, are now randomly selected 168 cases of the results reported below. 1 Materials and Methods 1.1 PHA Department of Guangzhou Institute of Pharmaceutical Industry freeze-dried injection. Diluted with saline, intradermal injection of 0.1ml (20ug) in the right forearm flexor side. After injection, the diameter of the pits was between 7mm and 9mm. After 24 hours, the diameter of the pits increased to (+) and decreased to (±) and disappeared to (-) ~ ([1]). 1.2 Mycobacterium tuberculosis PPD-C Department of Beijing Hi-Tech Life Science and Technology Development Corporation dilution. Intradermal injection of 0.1ml (50IU / ml) in the left forearm flexor. The results were based on the diameter of local tumor in 72 hours. No reaction was found when the diameter of tumor was less than 4 mm (+), the positive reaction (+) was 5mm ~ 10mm, the positive reaction was 11mm ~ 20mm (++) Although not exceeding this value, there was blistering and necrosis, which was a strong positive reaction (+++) to (2).