论文部分内容阅读
Human peripheral blood lymphocyte subpopulations were identified by immunofluorescence withlabelled monoclonal antibodies against surface antigens (CD3/CD19, CD3/CD16/CD56 and CD4/CD8) and T, B, NK and T subsets were scored by flow cytometry on a FACScan. Blood samples were irradiated with 25-250 mGy X-rays and cultured for 72 hours in RPMI 1640 medium supplemented with fetal calf serum. It was found that the percentage of total and helper T cells as well as the helper to suppressor T ratio increased with time at the expense of B and NK cells while the suppressor T subset was kept around the normal level. The general pattern of the change with time in culture was not significantly influenced by low dose radiation within 0. 1 Gy. There was no prcferntial deletion of the suppressor T subset with the doses used in the present study. Combining previous data with similar observations on the T subsets in immune organs and peripheral blood in mice after low dose whole-body irradiation, the authors emphasized t
Human peripheral blood lymphocyte subpopulations were identified by immunofluorescence with labeled led monoclonal antibodies against surface antigens (CD3/CD19, CD3/CD16/CD56 and CD4/CD8) and T, B, NK and T subsets were scored by flow cytometry on a FACScan. Were irradiated with 25-250 mGy X-rays and cultured for 72 hours in RPMI 1640 medium supplemented with fetal calf serum. It was found that the percentage of total and helper T cells as well as the helper to suppressor T ratio increased with time at The expense of B and NK cells while the suppressor T subset was kept around the normal level. The general pattern of the change with time in culture was not significantly influenced by low dose irradiation within 0. 1 Gy. There was no prcferntial deletion of the Suppressor T subset with the doses used in the present study. Combining previous data with similar observations on the T subsets in immune organs and peripheral blood in mice after low agent whole-body irradiation, the au Thors emphasized t