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目的探讨抗氧化剂普罗布考、AT1受体拮抗剂厄贝沙坦对人脐静脉内皮细胞(HUVEC)氧化损伤及PAI-1含量的影响。方法采用H2O(2100μmol/L)制作氧化应激损伤的细胞模型,给予不同浓度普罗布考、厄贝沙坦预孵育细胞:(1)空白对照组;(2)H2O2组;(3)Pro1组:H2O2+普罗布考(2.5μmol/L);(4)Pro2组:H2O2+普罗布考(5μmol/L);(5)Pro3组:H2O2+普罗布考(10μmol/L);(6)Irb1组:H2O2+厄贝沙坦(2.5μmol/L);(7)Irb2组:H2O2+厄贝沙坦(5μmol/L);(8)Irb3组:H2O2+厄贝沙坦(10μmol/L);(9)Pro3+Irb3组:H2O2+普罗布考(10μmol/L)+厄贝沙坦(10μmol/L)。用MTT法检测普罗布考及厄贝沙坦对HUVEC氧化损伤的影响;用ELISA方法测定培养液中PAI-1含量。结果 MTT:空白对照组、Pro2组、Pro3组、Irb3组、Pro3+Irb3组吸光度值高于H2O2组(空白对照组、Pro2组、Pro3组、Pro3+Irb3组P<0.01,Irb3组P<0.05);空白对照组、Irb1组、Irb2组和Irb3组培养液中PAI-1均高于H2O2组(空白对照组、Irb2组、Irb3组P<0.01;Irb1组P<0.05);与Pro+Irb组比较,H2O2组、Pro3组细胞培养液中PAI-1水平均较高(P<0.01)。结论普罗布考和厄贝沙坦可通过不同机制减轻氧化应激对HUVEC的影响,从而对血管内皮细胞产生保护作用;二者在改善氧化应激引起的PAI-1水平升高方面存在一定的协同作用。
Objective To investigate the effects of antioxidants probucol and AT1 receptor antagonist irbesartan on oxidative damage and PAI-1 in human umbilical vein endothelial cells (HUVECs). Methods Cell models of oxidative stress injury were established by H2O (2100μmol / L), and probucol and irbesartan were preincubated at different concentrations: (1) blank control group; (2) H2O2 group; (3) Pro1 group (4) Pro2 group: H2O2 + probucol (5μmol / L); (5) Pro3 group: H2O2 + probucol (10μmol / L) H2O2 + irbesartan 2.5μmol / L; Irb2 group: H2O2 + irbesartan 5μmol / L; Irb3 group: H2O2 + Irbesartan 10μmol / L; + Irb3 group: H2O2 + probucol (10μmol / L) + irbesartan (10μmol / L). The effect of probucol and irbesartan on the oxidative damage of HUVEC was detected by MTT assay. The content of PAI-1 in culture solution was determined by ELISA. Results Compared with H2O2 group, the absorbance of MTT group, Pro2 group, Pro3 group, Irb3 group and Pro3 + Irb3 group was significantly higher than that of H2O2 group (P <0.01, P <0.05 ); PAI-1 in blank control group, Irb1 group, Irb2 group and Irb3 group were higher than H2O2 group (blank control group, Irb2 group, Irb3 group P <0.01; Irb1 group P <0.05) Compared with the control group, the levels of PAI-1 in H2O2 group and Pro3 group were higher (P <0.01). Conclusions Probucol and irbesartan can reduce the effects of oxidative stress on HUVEC through different mechanisms and thus have a protective effect on vascular endothelial cells. Both of them have some effects on improving the level of PAI-1 induced by oxidative stress Synergy.