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目的探讨肿瘤抑制基因ｐ１６在几种类型白血病中的改变及其频率。方法对７７例原发性白血病及非霍奇金淋巴瘤标本分别采用Ｓｏｕｔｈｅｒｎ印迹杂交，ＰＣＲ┐ＳＳＣＰ的方法检测缺失及点突变。结果Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交发现ｐ１６基因异常共１１例，其中纯合缺失７例，异常重组片段４例，ＳＳ┐ＣＰ发现ｐ１６第三外显子异常泳动带６例，是否为突变有待测序证实。结论ｐ１６基因改变主要集中在急性淋巴细胞性白血病，占４７．７％（９／１９），提示这一肿瘤抑制基因在淋巴细胞发生恶性变的机制中起一定作用
Objective To investigate the changes and frequency of tumor suppressor gene p16 in several types of leukemia. Methods Seventy-seven primary leukemia and non-Hodgkin’s lymphoma specimens were detected by Southern blot hybridization and PCR-SSCP methods to detect deletions and point mutations, respectively. Results A total of 11 cases of p16 gene abnormalities were detected by Southern blot analysis. Among them, 7 were homozygous deletions, 4 were abnormally recombined fragments, and 6 cases were found abnormal movement of p16 exon 3 in SSCP. Whether mutations were to be confirmed by sequencing. Conclusion The p16 gene mutation is mainly concentrated in acute lymphoblastic leukemia, accounting for 47.7% (9/19), suggesting that this tumor suppressor gene plays a role in the mechanism of malignant transformation of lymphocytes.