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目的探讨乌鲁木齐地区支气管哮喘儿童ADAM33基因V4位点多态性、维生素D(VitD)水平及其相关性研究。方法选取同期3~15岁于乌鲁木齐地区生活的哮喘患儿154人,同时设立对照组健康非哮喘儿童120人,首先采用PCR聚合酶链反应对ADAM 33基因V4位点进行单核苷酸多态性(SNP)分型,并用ELISA法检测VitD水平,进一步根据民族进行组内分层,进行V4位点、VitD分析并进行相关性分析。结果 1)V4位点3种基因型频率分布在病例组和对照组间差异有统计学意义(χ~2=6.806,P=0.033),在不同民族间差异无统计学意义(实验组:χ~2=0.714,P=0.699;对照组:χ~2=5.898,P=0.052)。2)VitD水平检测病例组与对照组比较,差异有统计学意义(t=2.341,P=0.020)。结论1)ADAM33基因V4位点与乌鲁木齐地区支气管哮喘儿童发病有相关性,但无民族差异。V4位点突变后的G基因可能是支气管哮喘发病的危险因素。2)VitD水平与支气管哮喘儿童发病有相关性。
Objective To investigate the V4 polymorphism of ADAM33 gene and the level of vitamin D (VitD) in children with bronchial asthma in Urumqi and their correlation. Methods A total of 154 asthmatic children aged 3-15 years in Urumqi were enrolled in the study. At the same time, 120 non-asthmatic children were enrolled in the control group. PCR-based polymerase chain reaction (PCR) was used to detect single nucleotide polymorphisms (SNP). The level of VitD was detected by ELISA, and stratified intra-group according to ethnic groups. V4 locus and VitD were analyzed and the correlation was analyzed. Results 1) There was significant difference in frequency distribution of the three genotypes between V4 locus and control group (χ ~ 2 = 6.806, P = 0.033). There was no significant difference among different ethnic groups (experimental group: χ ~ 2 = 0.714, P = 0.699; control group: χ ~ 2 = 5.898, P = 0.052). 2) Compared with the control group, the difference between the VitD level test group and the control group was statistically significant (t = 2.341, P = 0.020). Conclusions1) The V4 locus of ADAM33 gene is associated with the incidence of bronchial asthma in Urumqi region, but there is no national difference. G gene after V4 mutation may be the risk factor of bronchial asthma. 2) VitD levels are associated with the incidence of bronchial asthma in children.