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本文报道了脂质体化白细胞介素6(IL-6)用作免疫佐剂的性能、以及与其他佐剂的比较结果。作者以65kDa热休克蛋白为模型抗原,在Balb/c小鼠体内比较了IL-6与其他佐剂的效果。 脂质体是由磷脂和其他极性两性物在水相中分散形成,由于它的弱免疫原性、低毒性和可生物降解等特性,因此它既是多种蛋白抗原的载体,又是免疫佐剂。此外,脂质体与某些有佐剂性质的细胞因子(如γ干扰素、白细胞介素1α等)结合,从而可增强免疫佐剂活性。游离形式的细胞因子由于在体内迅速被清除和失活,因而其应用受到限制。每天注射和提高剂量则会损害健康,且应用不便。将细胞因子结合到脂质体,形成一种缓释作用,则可排除上述不利因素。
This article reports the use of liposomal interleukin-6 (IL-6) as an immunoadjuvant and its comparison with other adjuvants. The authors compared the effect of IL-6 with other adjuvants in Balb / c mice using a 65 kDa heat shock protein as a model antigen. Liposomes are formed by the dispersion of phospholipids and other polar amphipathic substances in the aqueous phase. Due to their weak immunogenicity, low toxicity and biodegradability, liposomes are both carriers of various protein antigens and immune adjuvants Agent. In addition, liposomes bind to certain adjuvanted cytokines such as interferon-gamma, interleukin-1 alpha, etc., thereby enhancing immune adjuvant activity. Free-form cytokines are limited in their application due to their rapid clearance and inactivation in the body. Injecting and increasing doses daily can be detrimental to health and inconvenient to use. The combination of cytokines into liposomes to form a sustained-release effect can eliminate the above disadvantage.