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目的：了解围产期孕妇B族链球菌（GBS）的带菌情况及耐药性，为临床合理用药提供依据。方法对719例广东地区35～37周孕妇阴道或直肠分泌物标本，用选择性TH肉汤增菌培养基进行GBS培养，采用K-B法对分离的GBS进行药敏试验，并对红霉素耐药、克林霉素敏感的菌株进行D试验检测，分析其耐药状况。结果719例孕妇GBS检测阳性率为9．7％（70／719）；GBS对头孢噻肟、头孢曲松和利奈唑胺未发现耐药株，仅氯霉素和红霉素出现中介耐药菌株，中介率分别为2．9％和5．7％；对青霉素G、氨苄西林、头孢吡肟和万古霉素耐药率较低；对红霉素、克林霉素和左氧氟沙星的耐药率较高，分别为65．7％、60．9％和34．3％；对8株红霉素耐药而克林霉素敏感的GBS进行D试验检测发现1株阳性，D试验阳性率为12．5％。结论 GBS是广东地区围产期孕妇阴道携带的细菌之一，GBS对青霉素类和头孢菌素类抗菌药物的敏感性较高，而对大环内酯类、克林霉素和喹诺酮类抗菌药物的耐药性较强，此外，加做D试验可有效避免临床不合理使用克林霉素。“,”Objective To investigate group B Streptococcus (GBS) carrier rate in late pregnant women and drug susceptibility and provide information for clinical drug use. Methods 719 vaginal or rectal specimens were collected from 35~37 weeks pregnant women of Guangdong province and cultured in selective TH broth enrichment medium. Drug susceptibility tests of the isolated GBS were determined by the K-B method. The D-test was performed to the strains that were resistant to erythromycin and susceptive to clindamycin to analyze their drug resistance. Results The GBS carrier rate in the 719 pregnant women was 9.7%. No GBS strain was found to be resistant to cefotaxime, ceftriaxone and linezolid. Intermediate resistant strain was found to be resistant to chloramphenicol and erythromycin with a resistance rate of 2.9%and 5.7%, respectively. The resistance rates to penicillin G, ampicillin, cefepime and vancomycin were low. However, the resistance rates to erythromycin, clindamycin and levofloxacin were higher which were 65.7%, 60.9%, and 34.3%, respectively. There was one strain detected to be positive by D test out of 8 strains of GBS that resistant to erythromycin and susceptible to clindamycin. The positive rate of D test was 12.5%. Conclusion GBS is one of vaginal bacteria carried by perinatal pregnant women of Guangdong province. The strains are more sensitive to penicillins and cephalosporins, but are more resistant to macrolide, clindamycin and quinolones. In addition, application of D-test might effectively avoid the unreasonable clinical use of clindamycin.