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目的探讨实验性溃疡性结肠炎(UC)大鼠淋巴细胞过渡归巢现象与淋巴细胞表面CCR7受体表达的关系。方法40只SD大鼠随机分为对照组及模型组,观察结肠黏膜下淋巴细胞过渡归巢现象,逆转录-聚合酶链反应(RT-PCR)半定量法检测结肠组织中CCR7的表达;流式细胞法(FCM)测定结肠回流血液中CCR7+淋巴细胞的比例。结果模型组大鼠结肠黏膜下存在淋巴细胞过渡归巢,派尔集合淋巴结增生,最大截面积比较,差异有统计学意义[(9.56±0.32)比(1.48±0.58),t=5.12,P<0.01],CCR7 mRNA的表达量与对照组比较明显升高[(0.772±0.108)比(0.126±0.029),t=4.37,P<0.01]、模型组结肠静脉回流血中CCR7+淋巴细胞的比例明显高于对照组[(76.58±9.73)%比(16.62±7.84),t=3.97,P<0.01]。结论溃疡性结肠炎大鼠的结肠黏膜下存在淋巴细胞过渡归巢现象,结肠组织中归巢受体CCR7的表达增高,结肠回流血液中CCR7+的淋巴细胞的比例增高。
Objective To investigate the relationship between lymphocyte transitional homing and the expression of CCR7 receptor on lymphocytes in experimental ulcerative colitis (UC) rats. Methods Forty Sprague-Dawley rats were randomly divided into control group and model group. The transitional homing of colonic submucosal lymphocytes was observed. The expression of CCR7 in colonic tissue was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) The ratio of CCR7 + lymphocytes in colonic reflux blood was determined by flow cytometry (FCM). Results There were transitional homing of lymphocytes in colonic mucosa of rats in model group, Pall collection lymph node hyperplasia, maximum cross-sectional area, the difference was statistically significant [(9.56 ± 0.32) vs (1.48 ± 0.58), t = 5.12, P < 0.01]. The expression of CCR7 mRNA in the model group was significantly higher than that in the control group [(0.772 ± 0.108) vs (0.126 ± 0.029), t = 4.37, P <0.01]. The CCR7 + lymphocytes in the model group were significantly higher (76.58 ± 9.73)% vs (16.62 ± 7.84), t = 3.97, P <0.01]. Conclusion The transitional homing of lymphocytes in colonic mucosa of ulcerative colitis rats is observed. The expression of CCR7 in colonic tissue is increased, and the proportion of CCR7 + lymphocytes in colonic reflux blood is increased.