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目的 对各型肝炎病人血清和肝组织中输血传播病毒 (TTV)核酸检测分析 ,探讨病毒的致病性。方法 以地高辛为标记物制备TTVDNA探针 ,斑点杂交法、原位杂交法分别检测血清中及肝组织中TTVDNA。结果 检测 10 3例血清 ,TTV总阳性率为 2 5 2 4% (2 6 /10 3) ;甲～戊型肝炎组检出率 2 1 81% (12 /5 5 )、非甲～非庚型肝炎患者检出率 47 37% (9/19) ,显著高于正常对照组 15 % (3/2 0 )。临床可见TTV的单独感染和重叠感染 ;出现急性、慢性甚至重度肝损伤。 12例肝组织可见TTV阳性 ,阳性颗粒主要见于肝细胞核内。结论 从血清和肝组织证实了TTV的存在 ,提示这种病毒可能是导致肝脏炎症的一种新病原
Objective To detect and analyze the blood transfusion transmitted virus (TTV) in the serum and liver tissue of various types of hepatitis patients and to explore the pathogenicity of the virus. Methods TTVDNA probe was prepared using digoxin as a marker, Dot blot hybridization and in situ hybridization were used to detect TTVDNA in serum and liver tissue respectively. Results The positive rate of TTV was 105 2 4% (2 6/10 3) in 10 103 serum samples, and 21 81% (12/55) in hepatitis A and E, The detection rate of hepatitis B was 47 37% (9/19), which was significantly higher than that of the control group 15% (3/2 0). Clinical manifestations of TTV infection alone and overlap infection; acute, chronic and even severe liver injury. TTV positive in 12 cases of liver tissue can be seen, the positive particles mainly found in the nucleus of hepatocytes. Conclusion The existence of TTV was confirmed from serum and liver tissue, suggesting that this virus may be a new pathogen that causes liver inflammation