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目的探讨珍珠梅黄酮纳米粒对人肝癌Hep G-2细胞凋亡的影响。方法采用MTT法观察不同时间(24,48,72h)、不同浓度(50,100,200μmol/L)的珍珠梅黄酮纳米粒对人肝癌细胞(Hep G-2,Hep3B,PLC/PRF/5)及人正常肝细胞(Chang Liver)的增殖抑制作用;利用光学、荧光和透射电子显微镜观察Hep G-2细胞凋亡,并应用流式细胞术检测细胞凋亡情况。结果 MTT法检测细胞增殖抑制结果显示:不同浓度的珍珠梅黄酮纳米粒对人肝癌细胞(Hep G-2,Hep3B,PLC/PRF/5)及人正常肝细胞(Chang Liver)均有不同程度的抑制作用,对人肝癌Hep G-2细胞抑制最明显;形态学检测结果显示:珠梅黄酮纳米粒干预Hep G-2细胞48 h后,细胞出现明显的凋亡;流式细胞术检测阴性对照组、5-Fu阳性对照组及不同浓度珍珠梅黄酮纳米粒干预组(50,100μmol/L)凋亡率分别为为4.9%,89.6%,50.0%,86.2%。结论珠梅黄酮纳米粒可以抑制人肝癌Hep G-2生长并诱导其凋亡。
Objective To investigate the effects of pearl plum flavonoids nanoparticles on the apoptosis of human hepatoma Hep G-2 cells. Methods HepG-2, Hep3B, PLC / PRF / 5 cells were treated with different doses of pearl plummet flavones at different times (24,48,72h) and normal concentrations (50,100,200μmol / The proliferation of HepG-2 cells was observed by optical, fluorescent and transmission electron microscopy. The apoptosis of Hep G-2 cells was detected by flow cytometry. Results The results of MTT assay showed that different concentrations of pearl plummetron nanoparticles had different degrees of inhibitory effect on HepG-2, Hep3B, PLC / PRF / 5 and Chang Liver cells Inhibitory effect on Hep G-2 human hepatoma cells inhibited the most obvious; Morphological test results showed: Beomeral flavonoid nanoparticles intervened Hep G-2 cells 48 h after the cells showed significant apoptosis; flow cytometry negative control The apoptotic rate of the 5-Fu positive control group and the different concentrations of the pearl plummetone nanoparticle intervention group were 4.9%, 89.6%, 50.0% and 86.2%, respectively. Conclusion Bead-flavonoids nanoparticles can inhibit the growth of HepG-2 and induce its apoptosis.