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目的 :探讨NO的前体左旋精氨酸 (L arg)对急性心肌缺血 (AMI)大鼠心肌有无保护作用及其作用机制。方法 :采用垂体后叶素 (Pit)性急性心肌缺血模型 ,设立正常对照组、阳性对照组、及L arg治疗组 ,观察中性粒细胞 (PMN)表面L -选择素及心肌微血管内皮细胞表面P -选择素的表达 ,并测定心肌梗死面积、PMN浸润数及血浆NO、MDA的浓度。结果 :阳性对照组与正常对照组相比 ,其L -选择素及P -选择素表达上调 ,心肌PMN浸润数、血浆MDA的浓度明显升高 ,血浆NO的浓度降低。L arg治疗组上述指标改变的程度明显轻于阳性对照组 (P <0 .0 1 ) ,心肌梗死范围小于阳性对照组。结论 :外源性L arg对急性心肌缺血有防治意义 ,其作用的机制与NO抑制选择素的表达有关
AIM: To investigate the protective effects of L-arginine (NO), a precursor of NO, on myocardium of acute myocardial ischemia (AMI) in rats and its mechanism. Methods: The model of acute myocardial ischemia of Pit was established. The normal control group, positive control group and L arg treatment group were established. The expression of L - selectin and myocardial microvascular endothelial cells on neutrophil (PMN) The expression of P - selectin and the area of myocardial infarction, the number of PMN infiltration and the concentrations of plasma NO and MDA were measured. Results: Compared with normal control group, the expression of L - selectin and P - selectin in the positive control group was increased. The number of myocardial PMN infiltration, the concentration of plasma MDA and the plasma concentration of NO in the positive control group were significantly decreased. L arg treatment group the extent of the above indicators change was significantly lighter than the positive control group (P <0.01), myocardial infarction range less than the positive control group. CONCLUSION: Exogenous L arg has preventive and therapeutic effects on acute myocardial ischemia, and its mechanism of action is related to the inhibition of NO expression by selectin