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等电点是蛋白质最重要的物理化学性质之一.目前有多种测定等电点的方法.本文利用最简单的浊度/分光光度法和最先进的毛细管等电点聚焦法分别测定了河南牛皮明胶的等电点,对两种方法进行比较并论证了后一种方法的优势.
Isoelectric point is one of the most important physical and chemical properties of protein.At present, there are many methods to determine isoelectric point.In this paper, the simplest turbidity / spectrophotometry and the most advanced capillary isoelectric focusing method were used to determine the Henan Isoelectric point of leather gelatin, the two methods are compared and demonstrate the advantages of the latter method.