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利用响应面法优化黑番茄总黄酮的最佳提取工艺条件。在单因素试验的基础上,选取乙醇浓度、提取时间、提取温度及料液比为影响因子,应用Box-Behnken中心组合设计建立数学模型,以总黄酮提取量为响应值,进行响应面分析(RSA)。结果表明,最佳提取工艺条件为:乙醇浓度为73%,提取温度80℃,提取时间1.5h,料液比1:58g/mL,总黄酮提取率预测值为21.035mg/g,验证值为20.880mg/g,RSD=0.329%(n=5)。
Optimization of Extraction Conditions of Total Flavonoids from Black Tomatoes by Response Surface Methodology. On the basis of single factor experiments, ethanol concentration, extraction time, extraction temperature and the ratio of solid to liquid were chosen as influencing factors. A mathematical model was set up using the Box-Behnken central combination design. The response surface analysis RSA). The results showed that the optimal extraction conditions were as follows: ethanol concentration 73%, extraction temperature 80 ℃, extraction time 1.5h, solid-liquid ratio 1: 58g / mL, total flavonoid extraction rate 21.035mg / g, 20.880 mg / g, RSD = 0.329% (n = 5).