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探讨AngiostatinK( 1 3) [AK( 1 3) ]裸DNA治疗人脑胶质瘤的可行性和作用机制。用脂质体包埋法将AK( 1 3)基因的真核表达载体 pcDNA SAK( 1 3)注入裸鼠皮下SHG44人脑胶质瘤 ,观察肿瘤生长情况并计算抑瘤率 ,结合PCR、免疫组化、电镜、微血管和瘤体坏死区计数 ,确定抗血管生成基因治疗人脑胶质瘤的特点。AK( 1 3)裸DNA在荷瘤裸鼠瘤体内获得表达 ,裸鼠皮下胶质瘤血管生成能力显著下降 ,肿瘤生长受到抑制 ,抑瘤率达 43%。本研究证明 ,AK( 1 3)裸DNA能抑制人脑胶质瘤生长 ,为非病毒载体介导的抗血管生成基因治疗的应用奠定了基础。
To investigate the feasibility and mechanism of Angiostatin K (1 3) [AK (1 3)] naked DNA treatment of human glioma. The eukaryotic expression vector pcDNA-SAK (1 3) of AK (1 3) gene was injected into SHG44 human glioma subcutaneously in nude mice by liposome embedding method. The growth of tumor was observed and the tumor inhibition rate was calculated. Combined with PCR, Tissue, electron microscopy, microvessel and tumor necrosis area count to determine the characteristics of anti-angiogenic gene therapy of human glioma. AK (1 3) The naked DNA was expressed in tumor-bearing nude mice, the ability of angiogenesis in nude mice was significantly decreased, the growth of tumor was inhibited, and the tumor inhibition rate was 43%. This study demonstrates that AK (13) naked DNA can inhibit the growth of human glioma and lay a foundation for the application of anti-angiogenic gene therapy mediated by non-viral vectors.