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通过乙醇沉淀、透析、离子交换和凝胶色谱等分离方法,我们从白花蛇舌草中分离得到一种葡聚糖HD-27,ESI-MS检测其分子量为1800。利用化学及光谱分析方法进行结构研究,确定HD-27是由11个葡萄糖残基组成,其结构为α-D-G lc-(1→4)-[-αD-G lc-(1→4)]9-β-D-G lc。
A glucan HD-27 was isolated from Hedyotis diffusa by ethanol precipitation, dialysis, ion exchange, and gel chromatography. The molecular weight of the glucan was determined to be 1800 by ESI-MS. Structural studies using chemical and spectral analysis methods have confirmed that HD-27 is composed of 11 glucose residues and its structure is α-DG lc-(1→4)-[-αD-G lc-(1→4)] 9-β-DG lc.