论文部分内容阅读
目的:建立颐和春口服液中人参皂苷Rg1、Re、Rb1高效液相色谱法含量测定方法。方法:在Shim-packVP-ODS(150×4.6mm)色谱柱上,以流动相A:乙腈-甲醇-0.3%磷酸(10∶5∶85),流动相B:乙腈-甲醇-0.3%磷酸(50∶5∶45)梯度洗脱,波长203nm进行检测。结果:人参皂苷Rg1、Re、Rb1在0.04～0.4mg/ml范围内线性良好,加样回收率分别为96.0±3.2%、94.1±1.6%、96.2±3.3%,稳定性实验RSD分别为1.19%、2.85%、3.17%,重现性实验RSD分别为1.56%、1.97%、3.29%。结论:本法可用于颐和春口服液的质量控制。
Objective: To establish a HPLC method for the determination of ginsenoside Rg1, Re, Rb1 in Yihechun oral liquid. METHODS: The mobile phase A was acetonitrile-methanol-0.3% phosphoric acid (10: 5: 85) and the mobile phase B was acetonitrile-methanol-0.3% phosphoric acid 50:5:45) gradient elution at a wavelength of 203nm for detection. Results: The ginsenosides Rg1, Re, Rb1 showed good linearities in the range of 0.04-0.4 mg / ml. The recoveries of the samples were 96.0 ± 3.2%, 94.1 ± 1.6% and 96.2 ± 3.3%, respectively. The RSDs of stability were 1.19% , 2.85% and 3.17% respectively. The RSDs of reproducible experiments were 1.56%, 1.97% and 3.29% respectively. Conclusion: This method can be used for quality control of Yihechun oral liquid.