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应用免疫组化、原位杂交和Ｓｏｕｔｈｅｒｎ吸印技术，检测２０例ＨＢＶ抗原阳性的肝硬化、胃炎、溃疡病患者胃粘膜活检组织中的ＨＢｓＡｇ、ＨＢｃＡｇ及ＨＢＶＤＮＡ存在状况，并观察ＨＢＶ对胃粘膜组织学的影响。免疫组化显示，胃粘膜内ＨＢｓＡｇ和ＨＢｃＡｇ检出率分别为８５％及８０％；原位杂交技术ＨＢＶＤＮＡ检出率为５０％；Ｓｏｕｔｈｅｒｎ吸印技术检出游离型或整合型ＨＢＶＤＮＡ分别为４０％及５５％。表明胃粘膜内有ＨＢＶ抗原存在，ＨＢＶ可在胃粘膜内复制和整合。本发现ＨＢＶ引起胃粘膜组织损害的证据。需警惕ＨＢＶ血症者，胃粘膜损伤或活检创伤时，有导致医源性ＨＢＶ传染的可能。
Immunohistochemistry, in situ hybridization and Southern blotting were used to detect the presence of HBsAg, HBcAg and HBVDNA in gastric mucosal biopsy tissues from 20 HBV-positive patients with cirrhosis, gastritis and ulcer disease. The effects of HBV on gastric mucosal tissue The impact of learning. Immunohistochemistry showed that the detection rates of HBsAg and HBcAg in gastric mucosa were 85% and 80% respectively; in situ hybridization, the detection rate of HBVDNA was 50%; Southern blotting detection of free or integrated HBVDNA were 40% And 55%. The presence of HBV antigens in gastric mucosa indicates that HBV can be replicated and integrated in gastric mucosa. This discovery of evidence of gastric mucosal tissue damage caused by HBV. Need to be alert to those with HBV sepsis, gastric mucosal injury or biopsy trauma, there may lead to iatrogenic HBV infection.