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目的研究小干扰RNA(siRNA)靶向抑制趋化因子受体7(CCR7)表达对MG63人骨肉瘤细胞增殖、侵袭及凋亡的影响。方法设计并合成靶向抑制CCR7表达的siRNA,转入MG63细胞后,采用Western blot法检测CCR7蛋白水平,异硫氰酸荧光素标记的膜联素Ⅴ/碘化丙啶(annexinⅤ-FITC/PI)双标记结合流式细胞术检测细胞凋亡,MTT法检测细胞增殖,TranswellTM侵袭和迁移实验检测细胞侵袭和迁移能力。结果 MG63细胞转染CCR7-siRNA后,细胞CCR7表达明显下调;同时细胞增殖明显减弱,侵袭和迁移能力明显下降,且细胞凋亡率升高。结论下调MG63骨肉瘤细胞的CCR7,可明显抑制细胞增殖、侵袭和迁移,并能诱导细胞凋亡。
Objective To study the effect of siRNA on the proliferation, invasion and apoptosis of MG63 human osteosarcoma cells by inhibiting the expression of chemokine receptor 7 (CCR7). Methods siRNA targeting CCR7 expression was designed and synthesized. After transfected into MG63 cells, the level of CCR7 protein was detected by Western blot. The expression of AnnexinⅤ-FITC / PI was detected by fluorescein isothiocyanate ) Double labeling combined with flow cytometry to detect apoptosis, MTT assay cell proliferation, invasion and migration assay TranswellTM invasion and migration assay. Results CCR7-siRNA transfected MG63 cells significantly downregulated the expression of CCR7, while significantly decreased the cell proliferation, invasion and migration, and increased the apoptosis rate. Conclusion Down-regulation of CCR7 in MG63 osteosarcoma cells can significantly inhibit cell proliferation, invasion and migration and induce apoptosis.