研究表明,采用药丸植入、输注、注射和缓释乳剂法均能诱导啮齿类动物对吗啡产生机体依赖性。然而,常用的药丸植入法可导致小鼠产生约15%死亡率。本文应用药物混合饲料法(2 mg 吗啡/g 饲料,喂饲小鼠1或3天;1,2和3 mg 吗啡/g 饲料,喂饲小鼠,每3天更换一浓度,共9天)研究小鼠对吗啡的机体依赖性并测定吗啡处理小鼠的血浆和脑中吗啡含量。实验用雄性ICR 小鼠,体重23～26 g,10只一组。 Studies have shown that the use of pills implantation, infusion, injection and slow release emulsion method can induce rodents to produce morphine dependence. However, the commonly used pill implantations result in about 15% mortality in mice. In this study, mice were fed with 2 mg morphine / g diet for 1 or 3 days, 1, 2 and 3 mg morphine / g diet, and every 3 days for a total of 9 days) The body dependence of mice on morphine was studied and the morphine content in plasma and brain of morphine-treated mice was determined. Experimental male ICR mice weighing 23-26 g, 10 in one group.