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目的探讨粉防己碱在急性缺血性肾损伤中的保护性作用机制。方法采用动脉夹钳夹大鼠双侧肾蒂，造成双肾完全缺血４５分钟的动物模型，对粉防己碱用药及未用药组大鼠不同再灌注时间以及肾组织不同部位的肾小管上皮细胞损伤情况进行观察。结果在肾组织皮质区与外髓区缺血再灌注后的各时相内，用药组肾小管的损伤程度均明显低于未用药组（Ｐ＜０００１）；两组肾小管损伤程度在内髓区任何血再灌注时相内均无显著性差异（Ｐ＞００５）；两组均以外髓处肾小管形态学变化最为明显，其次为皮质、内髓的肾小管形态学变化最不明显。结论粉防己碱可减轻缺血再灌注后肾小管的损伤程度，它的主要作用部位可能在肾脏的皮质与外髓区域。
Objective To investigate the protective effect of tetrandrine on acute ischemic renal injury. Methods Arterial clamping of rats with bilateral renal pedicle, causing bilateral renal complete ischemia in animal models for 45 minutes on the tetrandrine medication and non-medication group of different reperfusion time and different parts of the renal tubular epithelial cells Damage was observed. Results In the renal cortex and extramedullary region after ischemia-reperfusion in each phase, the degree of renal tubular injury were significantly lower than the non-medication group (P <0001); the degree of renal tubular injury in the two groups were There was no significant difference in the reperfusion phase in the medullary region between the two groups (P> 005). The morphological changes of the tubules outside the medulla were the most obvious in both groups, followed by the morphological changes in the cortex and medulla Not obvious. Conclusion Tetrandrine can reduce the degree of renal tubular injury after ischemia-reperfusion, and its main role may be in the cortex and the extramedullary area of the kidney.