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V2O5 flower-like structures assembled by thin nanosheets were in-situ growth on ceramic tubes by hydrothermal process. The structural characterization indicates that V2O5 flower-like structures is orthogonal diamond phase, which entirely covered on the surface of ceramic tubes. TMA sensing measured results revealed that the sensor based on V2O5flower-like structures exhibited fast reversible and response, good selectivity to TMA and good stability at 200 ℃. The good sensing performance may be ascribed toflower-like structures and directly growth sensingfilm on the ceramic tube without structure damage. Our works give a simple in-situ growthflower-like structures route on sensing device, which exhibits potential application for detecting trace amounts of TMA gas.