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目的:建立垂盆草片的质量标准。方法:采用薄层色谱法,对垂盆草进行定性鉴别;采用HPLC法对槲皮素、山柰素及异鼠李素进行含量测定。结果:该薄层色谱法专属性强;槲皮素、山柰素及异鼠李素的线性范围分别为4.94~98.82μg·ml-1、4.94~98.82μg·ml-1、1.07~21.36μg·ml-1,r值均为0.999 9,平均回收率分别为103.1%、100.6%、100.1%(RSD分别为2.2%、2.0%、2.0%,n=6)。结论:该方法专属性强、准确、重复性好,可用作垂盆草片的质量控制方法。
Objective: To establish the quality standard of Prunella. Methods: TLC was used to qualitatively identify sodactylus. Quercetin, kaempferol and isorhamnetin were determined by HPLC. Results: The TLC was of high specificity. The linear ranges of quercetin, kaempferol and isorhamnetin were 4.94-98.82μg · ml -1,4.94 ~ 98.82μg · ml -1,1.07 ~ 21.36μg · Ml-1, r values were 0.999 9, the average recovery was 103.1%, 100.6%, 100.1% (RSD were 2.2%, 2.0%, 2.0%, n = 6). Conclusion: The method is specific, accurate and reproducible, and can be used as a quality control method for sowsprit.