An indicator system closely connected with eco-environment, in which indicator involves in such fields as society, economy, resources, environment and ecology, is first proposed for eco-environmental quality assessment.Then, a hierarchical model with four levels is established by virtue of these indicators and attributes. In the model,weights of indicators and attributes are determined by combining Delphi method with the Analytic Hierarchy Process (AHP) and a statistic method is used to eliminate the influences arising from the differences in dimension and magnitude of indicators. On these grounds, an AHP-statistics model is provided for regional eco-environmental quality assessment. As a case, such AHP-statistics model is utilized in the dynamic analysis of regional eco-environmental assessment in Chaohu Lake basin. Study results show that natural environmental quality in the watershed was in the declining state while social environmental quality was in a markedly improved situation from 1996, and the synthetic eco-environmental quality was gradually and slowly improved under the common influences of both natural and social environmental factors. Example of application testified the capacities of above methodology to evaluate the real and dynamic state of regional eco-environmental quality.