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目的:探讨老龄大鼠脑缺血再灌注后海马神经元超微结构变化,以及线粒体形态改变程度及性质。方法:建立老年大鼠脑缺血动物模型,正常对照组(A组)、缺血30min再灌注6h(B组)、12h(C组)、24h(D组)、48h(E组)和7d组(F组),用透射电镜观察海马神经元的超微结构变化,通过计算机图像分析系统对线粒体形态计量分析。结果:B组海马神经元超微结构与A组相同,E组和F组海马神经元损伤较重;E组和F组线粒体体密度、数密度、比表面和嵴膜密度较A组明显减少(P<0.05),线粒体平均体积和平均截面积较A组明显增大(P<0.05)。结论:老龄大鼠脑缺血后产生延迟性神经元死亡,其线粒体的形态结构也发生明显变化,这些变化直接与脑缺血再灌注时程相关。
OBJECTIVE: To investigate the ultrastructural changes of hippocampal neurons and the degree and nature of mitochondrial morphological changes in aged rats after cerebral ischemia-reperfusion. Methods: The model of cerebral ischemia in aged rats was established. The normal control group (group A), ischemia reperfusion 6h (group B), 12h (group C), 24h (group D), 48h Group F (F group). The ultrastructural changes of hippocampal neurons were observed by transmission electron microscopy. The morphology of mitochondria was quantified by computer image analysis system. Results: The ultrastructures of hippocampal neurons in group B were the same as those in group A, while the hippocampal neurons in groups E and F were more damaged. The density, number density, specific surface area and crest densities of mitochondria in groups E and F were significantly lower than those in group A (P <0.05). The mean mitochondrial mean volume and average cross-sectional area were significantly higher than those in group A (P <0.05). CONCLUSION: Delayed neuronal death occurs in aged rats after cerebral ischemia, and the morphological changes of mitochondria also change significantly. These changes are directly related to the cerebral ischemia-reperfusion course.