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目的:探讨深圳地区流行TTV病毒的主要基因型、基因变异状况。方法:采集长期维持性血液透析患者,以及非甲-非庚但血清ALT升高肝炎患者血清标本,提取病毒DNA后,巢式PCR扩增TTV ORF1部分基因,克隆、测序后,进行基因分型及基因变异分析。结果:对21例 TTV ORF1部分基因进行序列分析,基因同源性为60.3%~98.5%。21例TTV分离株中,G1型17例,其中,Gla亚型16例,G1b亚型1例;G2型 4例,其中G2a亚型0例,G2b亚型3例,另一例可能为新的亚型,命名为G2x。结论:深圳地区血液透析患者及非甲-非庚、但血清ALT升高患者流行TTV病毒的基因型主要为G1a亚型。TTV基因变异可这39.7%。
Objective: To investigate the main genotypes and genetic variations of the popular TTV virus in Shenzhen. Methods: Serum samples from hepatitis patients with long-term maintenance hemodialysis and non-A-non-G serum but elevated serum ALT levels were collected. After the viral DNA was extracted, part of the TTV ORF1 gene was amplified by nested PCR, cloned and sequenced, genotyped And gene mutation analysis. Results: The sequence analysis of 21 TTV ORF1 genes showed that the homology was 60.3% -98.5%. Among the 21 TTV isolates, there were 17 cases of G1 type, including 16 cases of Gla subtype and 1 case of G1b subtype; 4 cases of G2 subtype, 0 cases of G2a subtype and 3 cases of G2b subtype. Another case may be new Subtype, named G2x. Conclusion: The prevalence of TTV virus in hemodialysis patients and non-A-non-G hemodialysis patients in Shenzhen area is mainly G1a subtypes. TTV gene mutation can be 39.7%.