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目的:了解苏州地区儿童呼吸道人类博卡病毒(hBoV)混合感染的情况及混合感染后的临床特征。方法:选取2012-2015年苏州大学附属儿童医院呼吸科因呼吸道感染住院的患儿8 711例,收集鼻咽分泌物,应用实时PCR检测hBoV,逆转录-聚合酶链反应法(RT-PCR)检测人类偏肺病毒(h MPV)、人类鼻病毒(hRV),直接免疫荧光法检测呼吸道7种常见病毒包括呼吸道合胞病毒(RSV)、流感病毒A(InfA)、InfB、副流感病毒1型(PinfⅠ)、PinfⅡ、PinfⅢ、腺病毒(ADV)。结果:8 711例标本中,hBoV阳性358例(4.11%)。358例hBoV阳性患儿中,92例(25.70%)存在混合感染,常见混合的病原为hRV、RSV和PinfⅢ。与单纯hBoV感染患儿相比,hBoV混合感染患儿月龄小(15.8个月vs 20.3个月)、入院前病程长(12.1 d vs 9.5 d)、喘息发生率高(63.04%vs 48.12%)(P均<0.05),但两组患儿气促、呼吸困难、紫绀及住院时间比较差异均无统计学意义(P均>0.05)。结论:苏州地区儿童hBoV存在一定比例的混合感染,但混合感染并不加重病情。
Objective: To investigate the mixed infection of respiratory tract human Boca virus (hBoV) in Suzhou area and the clinical features after mixed infection. Methods: A total of 8 711 children hospitalized for respiratory tract infections in Children’s Hospital of Soochow University from 2012 to 2015 were enrolled in this study. Nasopharyngeal secretions were collected and detected by real-time polymerase chain reaction (RT-PCR) Seven common viruses in the respiratory tract, including respiratory syncytial virus (RSV), influenza A (InfA), InfB, and parainfluenza virus type 1, were detected using hMPV, hRV and direct immunofluorescence assays (PinfⅠ), PinfⅡ, PinfⅢ, adenovirus (ADV). Results: Of 8 711 specimens, hBoV was positive in 358 cases (4.11%). In 358 cases of hBoV-positive children, 92 cases (25.70%) had mixed infection. The commonly mixed pathogens were hRV, RSV and PinfⅢ. Compared with children with hBoV infection, children with hBoV infection had a small age (15.8 months vs 20.3 months), a long duration of admission (12.1 days vs 9.5 days) and a high incidence of wheezing (63.04% vs 48.12%), (P <0.05). However, there was no significant difference in breathlessness, dyspnea, cyanosis and length of hospital stay between the two groups (all P> 0.05). Conclusion: There is a certain proportion of mixed infection in hBoV in children in Suzhou area, but mixed infection does not aggravate the disease.