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目的 了解腹腔开放伤后海水浸泡对机体血浆中自由基水平的影响。方法 ２８只犬随机均分为对照组（Ａ组）、单纯腹部浸泡组（Ｂ组）、腹腔等渗盐水浸泡组（Ｃ组）和腹腔海水浸泡组（Ｄ组）４个组。Ａ组为单纯腹外伤，Ｂ组为直接将犬无伤腹部浸入海水中浸泡处理，Ｃ、Ｃ两组犬致伤后分别置入等渗盐水或海水中浸泡处理，观察血浆中丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）、超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）及血浆渗透压的变化。结果 犬腹腔开放伤并海水浸泡后 ６０ ｍｉｎ时，血浆中 ＭＤＡ与血浆渗透压水平显著升高（与浸泡前及 Ａ、Ｂ、Ｃ组比较，Ｐ＜０．０１），并随浸泡时间延长呈升高趋势；而同时 ＳＯＤ水平则显著降低（与浸泡前及 Ａ、Ｂ、Ｃ组比较，Ｐ＜０．０１），并随浸泡时间延长呈降低趋势。浸泡处理前后Ａ、Ｂ、Ｃ组间差别未见有显著性（Ｐ＞０．０５）。结论 腹腔开放伤区海水浸泡可引起机体自由基水平升高。
Objective To investigate the effect of seawater immersion on plasma free radical levels after abdominal open wounds. Methods 28 dogs were randomly divided into 4 groups: control group (group A), simple abdominal immersion group (group B), intraperitoneal saline infusion group (group C) and intraperitoneal saline immersion group (group D). Group A was treated with abdominal trauma only. Group B was immersed in sea water without injury, and dogs in groups C and C were immersed in isotonic saline or seawater respectively to observe the plasma malondialdehyde malondialdehyde, MDA, superoxide dismutase (SOD) and plasma osmotic pressure. Results The levels of MDA and plasma osmolality were significantly increased at 60 min after immersion in open abdominal cavities and seawater (P <0.01 compared with before immersion and in groups A, B and C), and prolonged with soaking time (P <0.01 compared with that before soaking and in groups A, B and C), and decreased with the prolongation of soaking time. There was no significant difference between A, B, C groups before and after soaking treatment (P> 0.05). Conclusion The seawater immersion in the open abdominal area can cause the body’s free radical level to rise.