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建立反相高效液相色谱法测定酒石酸美托洛尔片中酒石酸美托洛尔的含量。方法:采用奥泰公司C18色谱柱(220 mm×4.6mm,5μm),醋酸盐缓冲液(取醋酸铵3.9g加水810ml溶解后,加三乙胺2.0ml,冰醋酸10.0ml,磷酸3.0ml,摇匀)-乙腈(824:146)为流动相,检测波长为275nm,流速为1.0ml/min,柱温为30℃。酒石酸美托洛尔在0.05～4mg.mL-1范围内呈良好的线性关系(r=0.99995)。酒石酸美托洛尔平均回收率分别为99.3%。结论:本法简便、准确,可用于酒石酸美托洛尔片中酒石酸美托洛尔的含量测定。
To establish an RP-HPLC method for the determination of metoprolol tartrate in metoprolol tartrate tablets. Methods: The chromatographic column (220 mm × 4.6 mm, 5 μm) of Altai Company was used. Acetate buffer (taking 3.9 g of ammonium acetate and dissolving in 810 ml of water) was added 2.0 ml of triethylamine, 10.0 ml of glacial acetic acid and 3.0 ml of phosphoric acid , Shake well) - acetonitrile (824: 146) as the mobile phase at a detection wavelength of 275 nm, a flow rate of 1.0 ml / min and a column temperature of 30 ° C. Metoprolol tartrate has a good linear relationship in the range of 0.05 ~ 4mg.mL-1 (r = 0.99995). Metoprolol tartrate average recovery rates were 99.3%. Conclusion: This method is simple, accurate and can be used for the determination of metoprolol tartrate in tablets of metoprolol tartrate.